We therefore gathered salient. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 2. 116 SpectraMax M5 and M5e Multi-Mode. 2. The test conditions are as follows: mobile phase: acetonitrile/H2O (0. The chamber contains springs that position the cuvette in the proper alignment for a read. MIC assay: After diluting colloidal nanoparticle solutions of varying concentrations in TSB, they were injected onto a 96-well plate with a strain of bacteria at a concentration of 10 6 CFU/mL. Lift up the lid over the chamber to insert or remove a cuvette. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. All configurations. Statistical. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. , contact Molecular. Record fluorescence emission data in kinetic mode for 2 min with 20 s between reads to ensure that interaction has reached equilibrium and take average. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 37. If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. 9000-0756. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted at 48 h after exposure to volasertib, genistein, or gefitinib and quantified by a NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE, USA). 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). . In. TYPICAL SAMPLE VALUES Sensitivity: The calculated minimal detectable dose (MDD) is 15. * Nunc. 05 mol/L hydrochloric acid and 0. Italic and boldface type are used for emphasis. Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. Fluorescence Microplate Reader Comparison Chart. 4) and cells were stained with 150 nM TSQ for 1 min. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Allows you to use EOS lenses on a micro four thirds camera M4/3 K&F concept lens adapter. FlexStation™ III. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf, Switzerland). Dagda Lab. For a complete list of the instruments supported by this release of the SoftMax. book Page 1 Wednesday, July 7, 2010 2:09 PMLuciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. SpectraMax® ABS. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal)0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Phillips5, Martin A. 0 compensates for most changes in environmental temperature or pressure. 1, BIO-RAD Laboratories). Optical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. Bioz Stars score: 98/100, based on 1 PubMed citations. Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). 0. With optimized reagents, option to operate the. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5. Full content visible, double tap to read brief content. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). . • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. MTT values at day 3 were normalized to day 0. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. To assess the effect of CAD drugs on lysosomal activity in BMdM, fluorescence analysis using the DQ Green BSA probe was performed. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). 6 reviews. 2. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. SpectraMax Microplate Readers. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. SpectraMax M4 Multi-Mode Microplate Reader. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. After incubation, the biofilms were washed with PBS until fully remove crystal violet solutions and then, solubilized by adding a 150 μL acetic acid. After preparation, wrap in foil (see Note 1). Eos - M4/3 lens adapter - Lenses - Victoria, British Columbia | Facebook. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. Detection modalities are shown in Table 1-1. Bioz Stars score: 98/100, based on 1 PubMed citations. The drawing shows a standard microplate of 15 mm height covered with a lid. Methods for the accurate and rapid screening of IVT conditions will facilitate RNA polymerase engineering, promoter optimization, and screening for new transcription inhibitor drugs. 11. The percentage of viability of each treatment group in different. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. The incubation system pre-incubated at 37°C for 3 min minutes and 2 μL fluorescent substrate FD (5 μM, final concentration) was added for fluorescence analysis in fully automated fluorescent microplate reader (SpectraMax M4, Molecular Devices) (Wang et al. Choose from a three- (M3), four- (M4), or Cables. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4Relative florescence units (RFU) were quantified on a plate reader (SpectraMax®; M4; Molecular Devices, Sunnyvale, CA) every 1 minute over a 10‐minute period at 37°C, using excitation and emission wavelengths of 328 and 420 nM, respectively. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. Fluoresbrite® fluorescent nanoparticles were analyzed by spectrofluorimetry where particles were excited at 441 nm wavelengths and emission intensity was recorded at 486 nm wavelengths. (Shanghai, China). The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples. Dettmer4, Wayne A. Data are from ≥5 independent experiments. 304 signal level saturation. Laboratory Freezers. Molecular Devices SpectraMax® series microplate readers. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. All experiments. 5. Choose from a three- (M3), four- (M4), orCables. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. 1 Measurement of Intestinal Permeability (In Vivo). FlexStation ®, SpectraMax. Background-subtracted data values (mean +/- SD) are graphed. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader MTT reduction - a tetrazolium-based colorimetric assay for cell survival and proliferation (Maxline Application Note #5) (PDF) Summary: The MTT assay measures cell respiration and the amount of formazan. 39. SpectraMax i3(x) SpectaTest ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. 3. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. 73. Cellular viability was assessed by using resazurin at 1. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. SPECTROMAXx with iCAL 2. ® reader to the computer. View specifications, prices, citations, reviews, and more. RESEARCH PAPER Effect of particle size and surface charge of nanoparticles in penetration through intestinal mucus barrier Sony Priyanka Bandi & Yashada Sanjay Kumbhar & Venkata Vamsi Krishna VenugantiThe Fibrinogen standard curve was prepared as described in Section 10. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. On day 2, in the morning weight the mice. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The activity of untreated cells was normalized to 100% and the treated cell activity was compared with that. The available phosphorus in the soil was leached by double-acid extraction (0. Microplate Readers include the M3, M4, exception is noted. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-Mode MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e. Residual. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Roelli1, Amandine Stoss1, Rebecca A. 2. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 2. Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. 1. Spectramax M4 Multi mode Microplate Detection System: Benchpro 4100 instrument card processing station: Muse Cell Analyzer: Veriti 96w Thermal Cycler: Small Animal Anaesthesia System: HPLCs (Analytical & Preparative) ACQUITY UPLC H-Class Bio: LC-MS/MS Q-tof 6540: Gas Chromatograph: GC-2014: Automatic Digital Polarimeter: FT-IR. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. However, traditional polyacrylamide gel electrophoresis (PAGE) and high. Table 1. . Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. 383 inches whereas the maximum allowed is 5. Mitochondrial respiration using high-resolution respirometry . Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Click Read. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. The absorbance was determined by a microplate reader (SpectraMax M4, USA) at 405 nm. 0. Vol 4, Iss 22, Nov 20, 2014 . SpectraMax® M4). 2. Figure 1. What types of plates can be used with StakMax? SBS Standard 96 and 384 well plates with plate height of 12-16 mm are recommended. StakMax Handling Stacker Base Unit. Next to the pushbutton is a green LED. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Interpolated concentrations of native CRP in human serum and plasma samples. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Plate chamber design of the SpectraMax category 1 microplate readers. Cells were exposed to drug solutions with. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. . • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Data analysis methods. . These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeThen, cells were stained with MitoSox Red (5 μM) for 30 min. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. Like all. Data was calculated subtracting OD440 recorded with the final OD600 values. 7% of the total test aerosol mass) was not included in the collection analysis. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. . Pentobarbital sodium and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St. VIEW DETAILS. 1 M Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Fluorescence Microplate Reader Comparison Chart. The MDD was determined by calculating the mean of zero standard replicates (n=24) and. Highly selective for dsDNA over ssDNA or RNA. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. VIEW DETAILS The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. 4. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax M2, and M2e: Secondary modes: BMG LABTECH: CLARIOstar PHERAstar FS PHERAstar Plus FLUOstar Omega POLARstar Omega PerkinElmer: EnSpire® Multimode Plate Reader EnVision Multilabel Plate. 90-0083 series): Excitation 530 nm,. . Spectramax M Series, supplied by Danaher Inc, used in various techniques. Table of Contents. Example: [Drawer]. The instrument reads all Plate sections in the experiment. When the user applies a set temperature of 37°C, the top is heated to 37. To report an issue with this product, Brief content visible, double tap to read full content. • Tel. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]. 314 low lamp level warning. The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). 72 MB. The SpectraMax M2 and M2e are versatile and reliable. 12. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. WARNING! The instrument weighs approximately 36 pounds (16. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. Background-subtracted data values (mean +/- SD) are graphed. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. Home; Lighting; Work lights; User manual. Examples: “Press carefully to engage,” “ Do not press down. qPCR The jejunum was sampled immediately after termination, snap frozen, andChoose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Description 1. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The following document is intended to demonstrate setup of this instrument. Indie Digital & Print Publishing. The combination index (CI) was calculated. 0 calibration logic, which needs only 5 minutes and a single sample per day rather than the 30 or more minutes needed by conventional analyzers. . (Fair Lawn, NJ, USA). 05 mg mL −1. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. 2 only). 5. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. ). The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. Following digestion, 200 µl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485 and 525 nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). However, dynamic changes in soil microbiota under different tree ages have received little attention. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. After incubation, the fluorescence intensity was measured using Spectramax M4 (Molecular Devices) (excitation: 380 nm emission: 460 nm). The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . All configurations. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Titration of human serum and plasma (heparin) within the working range of the assay. com. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. My List. To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The pathophysiology of such. SpectraMax® M4). 3. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax Spectrofluorometers. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Lower limits of detection for ATP determined using the ATPlite . Plot the fluorescence intensity versus the cell seeding number. The sample absorbance was read at 450 nm using a SpectraMax M4 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Bottom-reading is usually recommended for cell-based assay with adherent cells. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Then the formazan crystals were solubilized in dimethyl sulfoxide, and the absorbance was recorded at 572 nm wavelength using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). Plate Formats: 6, 12, 24, 48, 96, 384 Well Plates. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. NCI-60 cell panel screening was performed by the NCI/National Institutes of Health developmental therapeutics program (Bethesda, US). Page: 1/6. Images were taken using a fluorescence microscope (Nikon 80i, Japan). Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. TEM. org) with optimized instrument settings (Table 1) were used to generate and analyze data. FP assay do not perform well with protein:protein interactions. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). *Statistical significance: p < 0. Samples (2 mg) were mixed with potassium bromide in a 1:100 ratio before the analysis. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. , 2020b). GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. What do I do now? In our tests, the Greiner Bio-One Fluotrac™ 200 Microplate has shown lower background. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. 4. Hexokinase reagent (Eagle Diagnostics) was added and the reaction absorbance was read at 340 nm using a SpectraMax M4 multimode plate reader (Molecular Devices). The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. . After incubation, the absorbance value of all samples was measured at 517 nm on a Spectramax M4. A series of Toll-like receptor 7 (TLR7)-specific antagonists and extensive structural analysis reveal the open conformation of the receptor and the structural basis of TLR7 antagonism. Pierce™ BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. 8. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. , San Jose, CA) was. Cell viability (%) was calculated as the ratio of the number of living cells in treated samples to that of the control [15,30]. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. , 2011). Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). TEM. . To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. All associated StakMax® stackers. Other technologies such as imaging. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 4. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). The plate reader. Highly selective for dsDNA over ssDNA or RNA. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Description 1. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384) Optimized Guaranteed* 96-well (200 µL) 1. ITEM#: M4-MOL Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. ZERO BIAS - scores, article reviews, protocol conditions and more7. High tolerance for contaminants. 23, SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 23 PathCheck Pathlength Measurement Technology and Interfering Substances Any material that absorbs in the 900 nm to 1000 nm spectral region could interfere with PathCheck Pathlength Measurement Technology measurements. 7 cells.